摘要
目的 :探讨酪酸梭菌和婴儿型双歧杆菌对某些肠道致病菌的拮抗作用。方法 :将酪酸梭菌和婴儿型双歧杆菌单株菌及两株菌混合后分别与几株肠道致病菌混合接种于 GAM液体培养基中进行厌氧培养 ,通过平板菌落计数法计算肠道致病菌的菌数。结果 :经混合培养后肠道致病菌的菌数明显下降。结论 :酪酸梭菌 L CL16 6株和婴儿型双歧杆菌 L CL172株在体外能明显抑制几株肠道致病菌的生长繁殖。
The pure culture of Clostridium butyricum LCL166 strain alone ,B ifidobacterium infantis LCL172 (Bif,infantis LCL172)strain alone and mixed cultu res of C butyricum LCL166 and Bif infantis LCL 172 was mixed in vitr o with that o f E Coli O157:H7,Shigella flexneri F2a,Salmonella typhi H034 propot ionally in GA M culture medium respectively During anaerobic incubation,quantities of each en t erpathogens in the mixed cultures were investigate As a result,the amounts of E Coli O157:H7,Shigella flexneri F2a,Salmonella typhi H034 in mixed cult ures of C butyricum LCL 166 and Bif infantis LCL172 were significantly lower than t heir pure culture alone respectively
出处
《中国微生态学杂志》
CAS
CSCD
2001年第5期260-262,共3页
Chinese Journal of Microecology