摘要
目的 :探讨普乐可复 (FK5 0 6 )对肾脏的毒性及其机制。方法 :分别给小鼠腹腔注射生理盐水、FK5 0 6 5mg·(kg·d) -1和 10mg·(kg·d) -1,连续用药 7d后断头采血 ,检测血肌酐 (Cr)、尿素氮 (BUN)、血栓素 B2 (TXB2 )和 6 酮 前列腺素F1α(6 keto PGF1α) ,并剖腹取其肾脏行常规病理检查。结果 :FK5 0 6 5mg·(kg·d) -1组血清BUN、Cr、TXB2明显高于空白对照组 (P均 <0 0 5 ) ,6 keto PGF1α较之显著降低 (P <0 0 5 ) ;光镜下可见肾近曲小管上皮细胞混浊肿胀 ,肾小球萎缩不明显。FK5 0 6 10mg·(kg·d) -1组血清BUN、TXB2明显高于FK5 0 6 5mg·(kg·d) -1组 (P <0 0 5 ) ,6 keto PGF1α显著降低 (P <0 0 5 ) ;光镜下可见肾小球萎缩 ,纤维化 ,严重者肾小球结构消失 ,肾近曲小管上皮细胞混浊肿胀。结论 :FK5 0 6的肾毒性主要表现为肾小球和肾小管损伤后的肾功能下降 。
Aim:To study the nephrotoxicity and its mechanism of prograf(FK506).Methods:FK506 5 mg·kg -1 ·d -1 and 10 mg·kg -1 ·d -1 and physiological saline were injected into abdominal cavity of 3 groups of mice, repectively for 7 days, then the mice blood was taken for test of serum Cr?BUN?TXB2 and 6 keto PGF 1α ,and the kidneys were taken out for pathological examination Results:The levels of serum BUN,Cr and TXB2 in FK506 5mg·kg -1 ·d -1 group were obviously higher than those in control group( P <0 05),while 6 keto PGF 1α was markedly lower( P <0 05),and also proximal convoluted tubule epthelical cell presented swollen and turbid and no obviously atrophic glomeruli were observed.The levels of BUN?TXB2 in FK506 10 mg·kg -1 ·d -1 group were signficantly higher than those in FK506 5 mg·kg -1 ·d -1 group ( P <0 05),but 6 keto PGF 1α was lower ( P <0 05).Atrophic and fibrilized glomeruli were observed and in severe condition the glomeruli disppeared and epithelial cell became swollen.Conclusion: Nephrotoxicity of FK506 demonstrates as the renal dystemetion after the damage of glomeruli and tubule, and the mechanism is related with the injury of tubule epithelial cell.
出处
《河南医科大学学报》
北大核心
2001年第6期728-730,共3页
Journal of Henan Medical University
