摘要
目的 :研究纤支镜下取得的活检肺癌组织、支气管冲洗液中肺癌细胞的端粒酶活性 ,探讨其在肺癌诊断中的意义。方法 :应用银染— TRAP法 ,检测了 2 6例肺癌组织、4例良性肺疾病组织 ,相应 3 0例支气管冲洗液中的端粒酶活性表达。结果 :2 6例肺癌组织中 ,2 2例端粒酶表达阳性 ,阳性率为 84 .64%。 2 6例肺癌患者支气管冲洗液中 ,12例端粒酶表达阳性 ,阳性率 4 6.15 %。结论 :端粒酶可能成为肺癌早期诊断的重要标志物 。
objective:To study telomerase activity in lung cancer cells from biopsy and from bronchial washings by fibronchoscopy,and to investigatethe significance of telomerase in the diagnosis of lung cancer.MethodsTelomerase activity in 30 cases of lung tissues (26 carcinome,4 benign lesions) and in corresponding 30 cases of bronhial washings specimens were exanined by PCR-based silver staining TRAP assay. Results Twenty-two of 26 lung cancer samples were positive in tolomerase activity with 84.62% of positive rate;Twelve of 26 bronchial washing samples were positive in telomerase activity with 46.15% of positive rate.However.No telomerase activity in 4 benign lesions samples and corresponding bronchial washings.Conclusion Telomerase may be an important marker in early diagnosis and a breakthrough in the tratment of lung cancer.
出处
《黑龙江医药科学》
2001年第4期11-12,共2页
Heilongjiang Medicine and Pharmacy
关键词
肺癌
纤支镜
活检组织
支气管冲洗液
端粒酶
诊断
Lung Cancer
Fibrbrenchoscopy
Bioptic tissue
Bronchial washing
Telomerase
Diagnosis.
