摘要
建立了高效液相色谱法测定糖脂双降茶中淫羊藿甙含量的方法。采用ODS柱 ,以甲醇 水 (体积比为 60∶4 0 )为流动相 ,用紫外检测器于 2 70nm处检测。结果表明 ,淫羊藿甙的质量浓度在 0 1g/L～ 0 5 g/L范围内与色谱峰的峰面积呈良好的线性关系 (r =0 9993 ) ,回收率为 10 1 0 %～ 10 1 7% ,RSD为 1 6%～ 2 7% (n =3 )。方法快速、简便、准确 ,结果稳定 ,重现性好 。
Icariin in 'Tangzhi Shuangjiang Cha' was quantitatively determined by HPLC with ODS column, a mixture of MeOH H 2O(60∶40, V/V ) as mobile phase and UV detection at 270 nm There was a good linear relationship within the range of 0 1 g/L 0 5 g/L (r =0 999?3) The average recovery was 101 4%, RSD was below 2 7%( n =3) The method is rapid, simple, accurate and reproducible And the method is proposed to be used for quality control of 'Tangzhi Shuangjiang Cha
出处
《色谱》
CAS
CSCD
北大核心
2001年第4期365-366,共2页
Chinese Journal of Chromatography
