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抑制剂对不育系岗46及F_1种子萌发中淀粉酶活性的分析 被引量:3

ANALYSIS OF THE EFFECTS OF TWO INHIBITORS ON AMYLASE ACTIVITY IN THE STERILE LINE GANGYOU AND ITS F 1
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摘要 抑制剂A与B分别设4种浓度,对水稻进行了浸种发芽试验。结果表明,抑制剂A能使种子萌动,但不能生长;抑制剂B能抑制种子完全不萌动。但一旦去抑制以后,则不能再抑制种子的生长。用3.5-二硝基水扬酸法测定酶活性表明,抑制剂A的淀粉酶活性变化与对照相似,α-淀粉酶与β-淀粉酶比对照略低;抑制剂B的淀粉酶活性变化与对照差异显著,处理种子淀粉酶活性10天中均处于极低水平。抑制剂A以抑制剂种子萌发中后续酶合成为主,从而抑制生长;抑制剂B以抑制种子内存酶激活为主。 The seeds of the above-stated rice genotypes were pre-soaked and germinated in inhibitors A and B at four concentrations. The results showed that inhibitor A did not inhibit the seeds from germinating but the germinated seeds could not grow; and inhibitor B effectively inhibited the seeds from germinating, but it could not inhibit their growth after the inhibiting period was over. The curves for the dynamics of amylase activity determined by the DNS method showed that amylase activity of inhibitor A was similar to that of the control and α-amylase was slightly lower than β-amylase; amylase activity of inhibitor B was significantly different from the control; and seed amylase activity remained very low for 10 days. It is concluded that inhibitor A mainly ingibits enzyme synthesis during germination while inhibitor B inhibits the activation of the inactivated enzymes perviously stored in the seeds.
出处 《西南农业大学学报(自然科学版)》 CSCD 1999年第2期149-153,共5页 Journal of Southwest Agricultural University
关键词 抑制剂 水稻 淀粉酶 种子萌发 穗发芽 酶活性 岗46不育系 inhibitors hybridization-paddy amylases seeds gerrnination
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