摘要
测定了 1976年从江苏南京采集的 54株小麦赤霉病菌菌株对多菌灵的敏感性 ,EC50 值在 0 .2 6 17～ 0 .6 544 μg.m L-1之间 ,1983年在同一地点采集的 76株 ,EC50 值相应为0 .2 517～ 0 .70 50 μg.m L-1,而 1998年从浙江、湖北、上海、福建、安徽、江苏各地采集的 10 4株菌株 EC50 值为 0 .2 4 78～ 6 .4 574 μg.m L-1,表明湖北、上海、浙江等地田间已检测到小麦赤霉病菌抗多菌灵菌株。紫外光诱导分生孢子也获得了该病菌抗多菌灵突变株 ,其 EC50 值为14.1993μg.m L-1。抗药性突变体 JPR与敏感亲本菌株 JPS相比 ,在菌丝生长、产孢量方面无明显差异 ,但 JPR孢子萌发率为 57.1% ,而 JPS为 10 0 % ,而且 50 %孢子萌发的时间较野生亲本菌株滞后 12 h。 JPR产生脱氧雪腐镰刀菌烯醇 (DON毒素 ) 3.90μg.m L-1,而 JPS产生的DON毒素为 9.2 8μg.m L-1。
isolates of Fusarium graminearum collected from Nanjing in 1976, 78 isolates from the same plot in 1983 and 104 isolates from Shanghai, Fujian, Anhui, Jiangsu and Hubei in 1998 were detected for the sensitivity to carbendazim(MBC) with EC 50 0.2617~0.6544, 0.2517~0.7050, 0.2478~6.4574 μg·mL -1 , respectively. The result showed the mutants of MBC resistant existed in the field of Hubei,Zhejiang,Shanghai, etc. The mutant with EC 50 14.1993 μg·mL -1 was also obtained when its conidiospores were irradiated with UV ray. There were no significant difference in mycelia growth rate and sporulation between mutant JPR and its parents isolate JPS. The conidial germination percentage of JPR was only 57.1% while that of JPS was 100%. The 50% conidial germination of JPR lagged 12 hours behind JPS. JPR produced 3.90 μg·mL -1 deoxynivalenol (DON toxin) while JPS produced 9.28 μg·mL -1 .
出处
《农药学学报》
CAS
CSCD
2000年第4期22-27,共6页
Chinese Journal of Pesticide Science
基金
国家"九五"攻关课题!(96 - 0 0 5- 0 1- 0 2 - 0 5)
