摘要
目的 研制CPC/BMP复合人工骨 ,检测其理化特性及缓释作用。方法 制备CPC/BMP及CPC骨块 ,扫描电子显微镜观察表面结构。用材料力学实验方法测定不同凝固时间的抗压强度和弹性模量的变化。测定孔隙率和体外溶解度 ,并以庆大霉素为例研究了CPC作为载体的缓释作用。结果 结晶后的材料呈多孔状结构 ,BMP呈微球状分布在孔隙间。测定的孔隙率为 44.5 %。骨块在体外实验有一定的溶解度。最初 2 4h内 ,凝固时间与抗压强度和弹性模量成正相关 ,凝固 2h后抗压强度达到极限强度的 5 0 %以上。 2 4h基本达到最高强度 ,分别为 5 1.0MPa和 60 .6MPa。对庆大霉素的释放可持续 2 0d以上 ,2 0d累计释放出实验总量的 84.5 %。结论 材料多孔状结构利于骨生长 ,同时具有理想的力学强度、缓释作用和一定的溶解度 ,可望用于临床骨缺损的修复。
Objective CPC/BMP composite were constructed by combining CPC with BMP, and then to detect some of the main properties related to clinical use, including physical and extended chemical properties and extended releasing property. Methods The surface of CPC/BMP and CPC grafts were observed with scanning electron microscope. The compression strength and Young's modulus in different crystallizing time were measured by compressive load to failure test. The porosity and solubility in saline were determined by different means. To know the extended releasing property of CPC graft as delivery system, gentamicin was detected by HPLC. Results By scanning electron microscope, BMP was seen as microsphere distributing in the pores of the CPC/BMP composite. Porosity of the CPC/BMP composite was 44.5% and solubility in saline was preferable. The crystallizing time evidently affected the strength and fragility in the first 24 h. The strength was still more than half of the ultimate strength within 2 h. 24 h after crystallizing, the compression strength of CPC/BMP and CPC grafts were 48.9 MPa and 60.6 MPa respectively. As a delivery system, the CPC can release gentamicin at least for 20 d, 84.5% of total gentamicin was released in the first 20 d. Conclusion The porous structure of the composite was suitable for osteoanagenesis, the compression strength, porosity, solubility and extended releasing property suggest the CPC/BMP composite could be used as material for bone graft substitute.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第7期800-802,共3页
Journal of Third Military Medical University
