摘要
目的 观察不同磁场强度，不同作用时间的低频电磁场（ＬＦＥＭＦ）对培养的人脐静脉内皮细胞（ＨＵＶＥＣ）一氧化氮合酶（ＮＯＳ）活性表达的影响，以探讨ＬＦＥＭＦ用于经皮冠脉腔内成形术（ＰＴＣＡ）及支架植入（ＩＶＳ）术后冠脉再狭窄（ＲＳ）防治的意义。方法 分别用不同磁场强度，不同作用时间的ＬＦＥＭＦ作用于体外培养人脐静脉内皮细胞系ＥＣＶ３０４，行ＡＢＣ免疫酶染色法，图像分析法分析ＬＦＥＭＦ对ＨＵＶＥＣ的 ＮＯＳ表达的影响。结果 除６０ ｍＴ ２０ ｍｉｎ组，６０ ｍＴ ３０ ｍｉｎ组外，其余各实验组内皮细胞结构型 ＮＯＳ（ｅｃＮＯＳ）表达均较对照组增强，差异显著（Ｐ＜０．０５）．所有实验组与对照组诱生型 ＮＯＳ（ｉＮＯＳ）均无表达。结论ＬＦＥＭＦ增强了培养的ＨＵＶＥＣ的ｅｃＮＯＳ的表达、可能适用于ＲＳ的防治。
Objective To evaluate the effect of the low frequency electromagnetic field ( LFEMF) of different intensity and time on the expression of nitric oxide synthase( NOS) in human umbilical vein endothelial cells(HUVEC) and to discuss if LFEMF can be used in the prevention of the coronary artery restenosis after percutanuous transluminal coronary angioplasty( PTCA). Methods Human umbilical vein endothelial cells line (ECV304) were cultured in vitro with DMEM and 5 percent calf plasma. The HUVECs were cultured with LFEMF of different intensity and time respectively. ABC immunohistochemistry technique and image analyzing method were used to evaluate the effect of the LFEMIF on the expression of NOS. Result The expression of ecNOS in each group was more than the control group except 60 mT 20 mm group and 60 mT 30 mm group( P <0.05) There was no expression of iNOS in each group. Conclusion LFEMIF can enhance the expression of ecNOS in cultured HUVEC2.s and may be suitable in the prevention of the restenosis after PTCA.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2001年第5期288-290,共3页
Chinese Journal of Physical Medicine and Rehabilitation
