摘要
目的：研究肝素和氢化可的松以及二者联合应用对人脐静脉内皮细胞（ECV304细胞）和成纤维细胞（WI－38细胞）的生长抑制作用和细胞凋亡的影响，并比较普通肝素与低分子肝素作用的差异，探讨肝素和氢化可的松联合应用抑制血管生成的可能性。方法：将细胞在含有药物的培养液中孵育一定时间，观察其形态，用MTT法测定细胞的增殖抑制率，电镜下观察细胞凋亡情况和ELISA法定量检测细胞凋亡程度。结果：肝素和氢化可的松单独均可抑制ECV304细胞增殖，作用呈剂量依赖性；肝素对WI－38细胞亦有增殖抑制作用。肝素和氢化可的松联合应用具有协同作用。低分子肝素抑制作用强于普通肝素。经电镜观察和ELISA法检测，肝素和（或）氢化可的松没有明显诱导细胞凋亡作用。结论：肝素可抑制血管内皮细胞增殖但不具有特异性，且和氢化可的松具有协同作用。以上作用不是通过诱导细胞凋亡实现的。
Objective To study the proliferation－inhibiting and apoptosis－inducing effects of heparin and／or hydrocortisone to both human umbilical vein endothelial cell ECV304 and fibroblast WI－38.to compare the different effects between unfractionated and low－molecular－weight heparin.and to investigate the possibility of combined application of heparin and hydrocortisone in angiogenesis inhibition． Methods The different cells were cultured for a period of time in different kinds of culture fluid including different drugs． We observed the morphology of the cells under optic and electronic microscopes.and investigated the proliferation－inhibiting ratio and apoptosis degree by using MTT－cell proliferation detection and ELISA－cell death detection methods． Results Heparin or hydrocortisone inhibited ECV304 cell proliferation in a dose－dependent manner separately.so did heparin to WI－38 cells． Combination of heparin and hydrocortisone showed synergetic effect． And low－molecular－weight heparin showed powerful inhibition effects than unfrationated heparin． No obvious apoptosis－inducing effects of heparin and／or hydrocortisone was found． Conclusion Heparin inhibits the proliferation of vascular endothelial cells unspecifically and has a synergetic effect with hydrocortisone． The inhibition effect does not depend on apoptosis－inducing effect．
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2001年第6期418-421,共4页
Chinese Journal of Clinical Oncology
基金
天津市自然科学基金资助!993604911
