摘要
目的 建立二氢睾酮 (DHT)合成受抑制的SD大鼠模型 ,为研究DHT的作用及作用机理奠定基础。方法 将 6 6只SD雄性大鼠分为 :5周龄 (A组 ) 2 4只 ,10周龄 (B组 ) 2 0只 ,5 8周龄(C组 ) 2 2只。各周龄组均分为正常对照组和喂饲 5α 还原酶抑制剂保列治组 ,保列治剂量为 4.5mg·kg-1·d-1。模型建立后 4、10周分两次麻醉下抽取大鼠腔静脉血 ,放射免疫法分别测定睾酮(T)、游离睾酮 (FT)和DHT浓度。结果 4周时模型实验组与对照组DHT浓度 (ng/L)分别为A组 :46 .5 5± 15 .6 9、16 5 .2 1± 5 5 .40 ;B组 :5 0 .42± 16 .76、10 4.71± 5 9.2 9和C组 :5 6 .0 8± 9.5 0、10 9.93± 5 3.48。 10周模型时实验组与对照组DHT浓度 (ng/L)为A组 :34.32± 18.96、92 .71±2 3.44;B组 :39.38± 15 .11、110 .82± 36 .0 9和C组 :36 .5 9± 10 .11、10 4.41± 31.41。不同周龄大鼠4周、10周模型实验组均分别显著低于正常对照组 (P <0 .0 5 ) ,而T、FT差异无显著性 (P >0 .0 5)。DHT浓度测定结果稳定。结论 喂饲 4.5mg·kg-1·d-1的 5α 还原酶抑制剂保列治 4周即可在不同周龄的SD大鼠中制成DHT合成受抑制的动物模型 ,喂饲保列治 10周时模型同样稳定。
Objective To establish the SD rat models in which the serum concentrations of dihydrotestosterone (DHT) were depressed.Methods The study was conducted on 66 male SD rats (group A: 24 rats, 5 weeks old; group B: 20 rats, 10 weeks old and group C: 22 rats, 58 weeks old). They were subdivided into group A1, B1, C1 (intact control) and group A2, B2, C2 (treated with finasteride 4.5?mg·kg -1 ·d -1 , orally), respectively. Four and 10 weeks later, half of the rats were operated under anaesthesia intraperitoneally in each group. During the operation, blood samples were taken for the measurements of testosterone (T), free testosterone (FT) and dihydrotestosterone (DHT) by radioimmunoassay.Results The serum concentrations of DHT in the 4 week models were as follows (ng/L): group A2/groupA1: 46.55±15.69/165.21±55.40, group B2/group B1: 50.42±16.76 /104.71±59.29 and group C2/Group C1: 56.08±9.50/109.93±53.48; The concentrations of DHT in the 10 week models were as follows (ng/L): group A2/group A1: 34.32±18.96/92.71±23.44, group B2/group B1: 39.38±15.11/110.82±36.09 and group C2/group C1: 36.59±10.11/104.41±31.41. The serum concentrations of DHT were significantly lower in the rats of subgroups A2, B2, C2 than those in the rats of subgroups A1, B1, C1, respectively (P<0.05). There was no significant difference in T and FT between subgroups A1, B1, C1 and subgroup A2, B2, C2 respectively. The radioimmunoassay method we founded to determine the level of DHT was stable and reliable.Conclusion The SD rat models in which the serum concentrations of DHT are restrained can be developed when 5α reductase inhibitor, finasteride (4.5?mg·kg -1 ·d -1 ) is taken orally for 4 weeks or longer than 4 weeks in every age group.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2001年第5期420-421,共2页
Chinese Journal of Experimental Surgery
基金
浙江省卫生优秀人才专项基金 (1 998年 )
