摘要
关于大片段DNA序列测定的策略,文献报道者已为数不少。本文作者在研究马传染性贫血病毒(Equine infectious anemia virus,EIAV)表面抗原基因(cDNA)变异性时,曾用鸟枪法获得了原病毒及变异病毒基因的核苷酸序列(共2.9kb)。但是,在该法测序过程中,95％的序列资料在较短时间内即可获,得而所剩5％的序列资料却需化费较长时间才能得到。为了克服这一不足,我们又采用了Bal31删除法稍加修改的策略。
The nucleotide sequence of the envelope gene of EIAV was determined using modified Bal31 deletion strategy. The method consists of cloning the cDNA into pUC9 or M13mp19 vector followed by construction of a series of Bal31 deletion mutants. After screening, single stranded M13-insert DNAs are prepared and analyzed by using a universal primer and the dideoxy chain-termination method.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第2期194-196,共3页
Chinese Journal of Virology
关键词
删除法
表面抗原基因
核苷酸序列
Bal31 deletion Envelope gene of Equine infectious anemia virus DNA sequencing
