摘要
口蹄疫病毒(FMDv)VP1蛋白质141～160位氨基酸的肽段,是免疫活性肽,含20个氨基酸,用DNA合成仪合成这段免疫活性肽基因片段,共72bp,5＇端带有Eco RI切点,3＇端带有BamHI切点,利用Eco RI和BamHI切点将兔疫活性肽基因片段克隆到带肴1ac启动子的基因表达载体pWR590质粒中,以O型FMDV抗体做为特异的IgG,检查FMDV抗原活性肽基因片段的表达,从210个菌落中选到有明显表达的菌株8株。
The peptide corresponding to the 141-160 sequences of VP1 of foot and mouth disease virus is an immunogenic domain .It contains 20 amino acids. The gene fragment coding for this immunogenic peptide was chemically sy-thesized by DNA synthesizer. This fragment is 72bp long with Eco RI sites at the 5' end and a Bam HI sites at the 3' end.The synthetic nucleotide fragment was inserted into plasmid pWR590 which is an expression vector with lac promoter. Identification of the gene product was carried oat by using the antibody of O type of foot and mouth disease virus. 8 clones from 210 transformants were positive. The expressed product formed a fusion protein with β-galactosidase.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第1期41-45,共5页
Chinese Journal of Virology
关键词
口蹄疫病毒
VP1基因
基因克隆
Foot and mouth disease virus VPl Immunogenic peptide Cloning and expression
