摘要
利用已克隆的猪IgGⅡ类Fc受体cDNA(swFcγRⅡ)基因序列(DQ026064)设计的引物S29和S20,通过RT-PCR技术从猪外周血白细胞cDNA中扩增了完整的960bp swFcγRⅡ ORF序列。利用基因重组技术将swFcγRⅡ ORF基因成功亚克隆到真核表达载体pcDNA3中。然后用脂质体法转染COS-7细胞,用玫瑰花环试验鉴定了swFcγRⅡ受体的亲和特性。半定量PCR检测表明,swFcγRⅡmRNA在外周血白细胞、肝、肺和肠系膜淋巴结有较高表达。
Based on cloned sequence（DQ026064）of swine FcγRⅡ gene, primers S29 and S20 were designed. ORF sequence of 960 bp in size of swine FcγRⅡ gene was amplified from cDNA prepared from peripheral blood leucocyte with the designed primers S29 and S20 by RT PCR. The ORF sequence was subcloned successfully into the expression vector pcDNA3 and then transfected into COS 7 cells. The COS 7 cells transfected with the swFcγRⅡ cDNA were able to bind chicken erythrocytes sensitized with porcine IgG. Identification of swine FcγRⅡ would help to understand IgG-FcγR interactions,and to develope new immunization protocols. The semiquantitative PCR analyses indicated that swine FcγRⅡ gene was expressed in liver, lung, mesenteric lymph node and peripheral blood leucocytes（PBLs）.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2008年第9期791-795,共5页
Chinese Veterinary Science
基金
国家自然科学基金项目(30400323)
