摘要
1986年7月,从捕自云南省西双版纳地区的棕果蝙蝠脑组织中分离出1株病毒,经理化、生物学性状及血清学鉴定,为基孔肯雅病毒,此为我国首次报道,用血凝抑制试验测定人及动物血清基孔肯雅病毒抗体,健康人阳性率为6.93％(70/1010),恒河猴为2.45％(5/204),猪为4.08％(8/197),表明云南省人及动物中存在本病毒感染。
In July 1986, a strain of virus was isolated from a pool of brain tissues of bats(Rousettus leschenaulti ) collected from Xishuangbanna area, Yun-nan Province, China. This virus strain could regularly cause illness and death in suckling mice, produced evident CPE in the C6/36 and BHK21 cells. It agglutinated red blood cells of dove, goose and sheep at pH 5.75-6.4. This virus is sensitive to acid and ether but resistant to 5-FduR. It is a RNA virus.This virus has been identified serologically by hemagglutination inhibition ( HI ), immunofluorescent and neutralization tests. It showed specific reaction of high titres to Chikungunya ( CHIK ) viusr only and no reaction with Mayaro, Semliki forest disease, Sindbis, Getah, Sagiyama, M1, Dengue fever and Japanese encephalitis viruses. Therefore it is identified as Chikungunya virus. This is the first report isolation of CHIK vfrus in China's Mainland.Sera from normal human population and animals in Yunnan were tested for antibodies against CHIK virus by HI test with positive results of 6.93% (70/1010)for man, 2.45% (5/204)for Macaca mullata and 4.06%(8/197) for pigs. These results demonstrated that CHIK virus infection exists in human beings and animals in Yunnan Province.
出处
《病毒学报》
CAS
CSCD
北大核心
1989年第1期31-36,共6页
Chinese Journal of Virology
关键词
基孔肯雅病毒
蝙蝠
血清抗体
Chikungunya virus Bat Serological investigation
