摘要
作者从海南省儋县地区蚊虫和蝙蝠分离出的两株病毒,电镜观察为球形,直径为50～65nm,对2～3日龄小白鼠能致死,在C6/36白纹伊蚊细胞、BHK_(21)细胞组织培养中增殖并产生明显的病变,经理化和生物学鉴定,对酸,乙醚均敏感,属RNA病毒,经血清学鉴定为基孔肯雅病毒。
Two isolates (HN_(24) and HN_(38))of chikungunya virus isolated from culex fatigans and bats captured in zhan county, Hainan, are reported in this paper. Electron microscopic observation of the isolates showed that they were characterized by spherical enveloped alphavirus particles with a diameter of 50～65 nm.
The isolates could grow in C6/36 cells and EHK 21 cells, and showed marked CPE. They could induce illness, and were sensitive to acid and ether but resistant to 5-Fduk. Both the isolates were identified as chikungunya virus by immunofluorecent and neutralization tests.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
1993年第3期205-208,共4页
Chinese Journal of Vector Biology and Control
基金
国家自然科学基金
