摘要
以兔抗牛肺疫 C88004株血清制备5%协同凝集试验诊断液并用之于检测兔和牛霉形体感染,结果表明:4只人工感染 C88004株兔肺与气管浸出液阳性检出率分别为75%(3/4)和100%(4/4),20只教学用兔分别为20%(4/20)和0%,6只兔病毒性出血症病毒感染兔皆为阴性.采自牛肺疫疫区14例牛肺浸出液和胸水的阳性检出率分别为78.6%(11/14)和21.4%(3/14)。协同凝集试验用于检测牛肺疫病原,特异性强,重复性高,简便易行,值得推广。
5% Coagglutination Diagnosticum made by rabbit anti bovine M- ycoplasma C88004 strain serum was used in COAG test to detect M- ycoplasma Mycoides infection with rabbit and cattle.The results o- btained were as follows.The Detected rate of Lung and trachea of 4 rabbits infected with C88004 was respectively 75% (8/4) and 100%,(4/4),20 rabbits for teaching experiment 20% (4/20) and 0%,6 rabbits infected with RVHV all negtive.The detected rate of lung and pleural fluid of 14 cattle collected from epidemic area was respectively 78.6% (11/14) and 21.4% (3/14).This indicat- ed that COAG test was a speeial,sensitive and simple method in detecting Mycoplasma M.infeetion in cattle.
关键词
牛肺疫
霉形体
兔
协同凝集试验
诊断
Mycoplasma Mycoides
cattle
rabbit
coagglutination test
