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静水压休克法诱导三倍体鲶鱼(silurus asotus L)的研究 被引量:4

STUDY ON TRIPLOID OF CATFISH (Silurus asotus L.)INDUCED BY HYDROSTATIC PRESSURE SHOCK
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摘要 采用静水压休克法诱导三倍体鲶鱼。通过对受精时间、静水压力及持续施压处理时间三方面进行筛选试验的结果表明,鲸鱼卵受精4~5min,用600~640kg/cm2静水压力处理3min,可以获得100%的三倍体鲶鱼,而且胚胎存活率也较高,孵化率达对照组的90%以上,是静水压休克法诱导三倍体鲶鱼的最佳条件、三倍体鲶鱼的倍性用细胞遗传学方法验证。 Hydrostatic pressure shock was used to induce and produce triploid silurus asotus L. By a series of tests for the fertilized times,hydrostatic pressure and treated times of catfish eggs, the results indicated that the best condition of inducement triploid is 4-5 min after fertilization, and that the eggs were treated for 3min at 600~640kg/cm2 of hydrostatic pressure. Which not only led to 100% of triploid Silurus asotus L. but also resulted in relatively high survival percentage of embryos. The percentage of hatching was as 90% as that of the control. The triploidy of these fish was verified by cytogenetic methods.
出处 《水产学杂志》 CAS 1997年第1期10-13,共4页 Chinese Journal of Fisheries
关键词 静水压休克 鲶鱼 三倍体 人工诱导 受精时间 静水压力 持续施压处理时间 hydrostatic pressure shock,catfish,triploid
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