摘要
目的 探讨cag致病岛的左右两部分cagⅠ与cagⅡ在中国人群感染幽门螺杆菌 (Hp)中的分布差异及其与Hp致病的相关性。 方法 采用PCR方法检测了 10 7例临床分离培养的Hp菌株 ,分别扩增cag致病岛的cagⅠ中的 170bp及cagⅡ中的 5 97bp片段。 结果 cagⅠ和cagⅡ均阳性的检出率为 6 1.7% ;单纯cagⅠ或cagⅡ的阳性检出率分别为 2 5 .2 %、7.5 % ;cagⅠ和cagⅡ均阴性的检出率为 5 .6 %。 结论 cagⅠ阳性检出率明显高于cagⅡ ,其差异主要存在于慢性胃炎中 (P <0 .0 1) ,在消化性溃疡等疾病中的检出无显著差异 (P >0 .0 5 )
Objective To investigate the difference of cag Ⅰ and cag Ⅱ in distribution of Helicobacter pylori (Hp) isolated from Chinese patients, and its relationship to gastroduodenal diseases. Methods cag Ⅰ (170 bp) and cag Ⅱ (597 bp) in cag pathogenicity island were amplified by polymerase chain reaction (PCR) in 107 Hp strains from Chinese patients. Results The amplicom to cag Ⅰ was positive in 93 strains, and fragment to cag Ⅱ was positive in 74 strains, cag Ⅰ or cag Ⅱ was positive alone in 27 or 8 strains respectively. The distribution of cag Ⅰ and cag Ⅱ in Chinese Hp strains was different ( P <0.01). Conclusion The positivity of cag Ⅰ is obviously higher than that of cag Ⅱ in Chinese Hp strains, especially in Hp infected chronic gastritis.
出处
《上海医学》
CAS
CSCD
北大核心
2001年第8期478-480,共3页
Shanghai Medical Journal
基金
国家自然科学基金 (No .3 9670 64 8)
欧共体委员会科学基金 (IC18CT95 0 0 2 4)
