摘要
目的 :明确冬虫夏草寄主昆虫拟青霉病病原 ,发病状况 ,为防治提供依据。方法 :分别观察卵、幼虫、蛹、成虫各阶段发病情况 ;分别取血淋巴、僵虫体和分生孢子 ,进行分离培养 ,观察鉴定菌种 ;分离培养的纯菌种接种健康幼虫。结果和结论 :病原菌为粉状拟青霉Paecilomycesfarinosus。幼虫期发病严重 ,蛹发病较少 ,成虫及卵期未见发病。幼虫呆滞疲软为主要病状。死虫体表面后期所产出之白粉状分生孢子层为典型病征。
Objective: To Clarify the cause of the disease of Cordyceps sinensis host insect Paecilomyces muscardine. Method: Seperately observing eggs,larvals,pupals,adults and their circumstances causing diseases in every stage. Adopting seperately Hemolymph,bodies of dead insects and conidiospores; seperating and culturing them,them observing and identifying the inoculum. Inoculating healthy larvals from the purely seperted and cultured plants of inoculum .Resut and Conclusion: The original inoculum of the disease is Paecilongces frinoues, which may often be found in the period of larvals and seldom found in the period of pupals but never in that of adults and eggs. The main symptom of larval is dullness and weakness. But the typical aymptom is a layer of a kind of white power of conidiospore on the surface of the dead body which comes into being later stage. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2001年第7期455-456,共2页
China Journal of Chinese Materia Medica
基金
国家"八五"攻关资助项目 (85 92 0 )
