摘要
本文用BclⅠ、AvaⅠ、BamHⅠ、PstⅠ、KpnⅠ、PvuⅡ共 6种限制性内切核酸酶 ,分析了 15尾青海湖裸鲤mtDNA的限制性片段长度多态性 ,共检测出 2 0个酶切位点 ,发现BclⅠ、BamHⅠ和PvuⅡ三种酶切类型具有多态性。根据不同个体mtDNA的酶切类型 ,青海湖裸鲤存在 4种mtDNA单倍型 ,计算mtDNA多态度π值为 0 .0 0 43,初步认为青海湖裸鲤在线粒体DNA上存在较丰富的群体内变异。
An analysis of patterns of cleavage of mtDNA by restriction endonucleases was performed for 15 Gymnocypris przewalskii przewalskii .Mitochondrial DNA polymorphisms were detected in the restriction patterns generated by the following 6 enzymes, BclⅠ?AvaⅠ?Bam HⅠ? PstⅠ?KpnⅠ?PvuⅡ .Only 3 of them( BclⅠ?Bam HⅠ? PvuⅡ ) were found to be polymorphisms.Our results showed that there were 4 mtDNA haplotypes in Gymnocypris przewalskii przewalskii and the genetic divergenec(π)was 0.0043,which indicated that mtDNA genetic diversity in Gymnocypris przewalskii przewalskii is higher.
出处
《遗传》
CAS
CSCD
北大核心
2001年第5期445-448,共4页
Hereditas（Beijing)
基金
国家自然科学基金资助课题 (编号 :3986 0 0 6 0 )。
关键词
青海湖裸鲤
MTDNA
限制性片段长度多态性
遗传多样性
Qinghai lake Naked carps
mitochondrial DNA
restriction fragment length polymorphism(RFLP) analysis
genetic diversity
