摘要
目的 :检测体外受精妇女排卵时卵泡液中白血病抑制因子 (LIF)的水平 ,探讨其与卵泡发育及排卵的关系。方法 :应用双抗体酶联免疫吸附法 (ELISA)检测 45例体外受精妇女排卵时卵泡液和外周血中LIF的水平。结果 :①排卵时卵泡液中的LIF浓度 (2 2 77± 14 11)ng/L高于外周血中的浓度 (3 2 0±1 2 5 )ng/L(P <0 0 0 1)。②成熟卵细胞的卵泡液中LIF的浓度 (3 1 3± 15 90 )ng/L高于成熟前卵细胞的卵泡液中的浓度 (11 91± 9 13 )ng/L(P <0 0 0 1)。③成熟前卵细胞的卵泡液中的浓度 (11 91± 9 13 )ng/L高于外周血中的浓度 (3 2 0± 1 2 5 )ng/L(P <0 0 1)。结论 :LIF是诱导卵泡发育和排卵的重要细胞因子。
Objective:To detect the changes of concentrations of leukemia inhibitory factor of ovulatory follicular fluid in IVF females as well as to explore the relationship between LIF and follicular development and ovulation.Methods:Follicular fluid LIF and serum LIF concentrations in 45 IVF females were measured by sandwich enzymelinked immunoadsorbent assay(ELISA).Results:Follicular fluid concentrations of LIF〔(22.77±14.11)ng/L〕 were significantly higher than serum LIF concentrations 〔(3.20±1.25)ng/L〕 ( P<0.001). Follicular fluid LIF concentrations of mature oocyte 〔(31.13±15.90)ng/L〕were significantly higher than follicular fluid LIF concentrations of premature oocyte 〔(11.91±9.13)ng/L〕( P<0.001) .Conclusion:LIF may be a important cytokine in inducing follicular development and ovulation.
出处
《山东医科大学学报》
2001年第4期348-349,共2页
Acta Academiae Medicinae Shandong
关键词
体外受精
卵泡液
排卵
细胞因子
白血病抑制因子
Fertilization,in vitro
Follicular fluid
Ovulation
Cytokine
Leukemia inhibitory factor
