摘要
目的研究脑毒清胶囊对大鼠脑组织缺血再灌注后c-fos基因表达和病理变化的影响。方法采用线栓法制成大鼠大脑中动脉缺血再灌注模型,免疫组化法测定并图像分析c-fos基因表达,光镜观察神经元损伤情况。结果缺血再灌注模型栓塞侧皮层及海马区c-fos基因表达显著增多,镜下观察神经元损伤最严重,而脑毒清大小剂量组c-fos基因的表达显著低于模型组P<0.01,正常神经元率显著高于模型组P<0.01。结论脑缺血再灌注后脑组织的c-fos基因表达显著增多,脑毒清胶囊能抑制缺血后c-fos基因表达,这可能是防治中风先兆取得较好疗效的机理之一。
Objective:To study the effect of Naoduqing capsule on the c -fos gene expression and pathomorphology in rats with injury of cerebral is-chemia -reperfusion.Methods:Rat models of middle cerebral artery occlusion(MCAO)were made by suture method.The assay and image pattern analysis of the c -fos gene ex pression were performed by im-munohistochemical method and the in jury of neuron was observed by optical microscope Results:The c -fos gene expression in the cortex and hippocampus of the occlusion side of the model group increased markedly,and the injury of neuron wa s most serious,while c -fos gene expression of the big and small doze g roups was remarkably lower than that of the model group(P<0.01)and the rate of normal neuon was significantly higher than that o f the model group(P<0.01).Conclecsion:C -fos gene expression of the cerebral tissues with injury of cerebral ischemia -reperfusion enhanced markedly,while Naoduqing capsule could inhibit the c -fos gene expression,which might be one of the mechanisms for it t o prevent and treat effec-tively threatened apoplexy.
出处
《中国中医急症》
2001年第4期227-228,共2页
Journal of Emergency in Traditional Chinese Medicine
基金
山东省教委资助课题No.J98K55
