摘要
目的 通过ＤＮＡ疫苗诱生乙肝病毒表面抗原（ＨＢｓＡｇ）特异性体液免疫应答。方法 将编码乙肝 病毒表面抗原的真核表达质粒免疫ＢＡＬＢ／ｃ小鼠，并分别于第１ｗ、第３ｗ后加强免疫１次，同时设对照组注射质粒 ｐｃＤＮＡ３．１。第２次加强免疫３ｄ后采血检测血清中 ＨＢｓＡｇ和 ＨＢｓＡｂ。结果 实验组２ｗ时检测到ＨＢｓＡｂ，２个月后达 到高峰。对照组未检测到 ＨＢｓＡｂ。实验组和对照组始终均未检测到 ＨＢｓＡｇ。结论 乙肝病毒 ＤＮＡ疫苗能引起小 鼠特异性体液免疫应答。
Objective To induce HBsAg specific humoral immunity by DNA vaccine. Methods BALB/c mice were immunized with the eucaryotic expression plasmid encoding HBsAg, using plasmid pcDNA3. 1 as control. Two boosters were given 1 and 3 weeks after immunization. Results HBsAb was detect-ed out in experimental group 2 weeks and reached a peak value 2 months after immunization, but no HBsAb was detected out in control group. However, neither HBsAg was detected out in the 2 groups. Conclusion Hepatitis B DNA vaccine can induce specific humoral immunity in mice.
出处
《中国生物制品学杂志》
CAS
CSCD
2001年第3期162-164,共3页
Chinese Journal of Biologicals
