摘要
目的 探索喉癌生物治疗新方法。方法 建立人喉癌裸鼠模型。瘤内注射新城疫病毒。对治疗后的组织进行病理学检查、病毒分离实验 ,血清ELISA实验。结果 在实验组与对照组之间 ,鼠重 (t′ =2 .397)、瘤重 (t′ =2 .85 2 )经t′检验P <0 .0 5 ,瘤体积 (t′ =6 .0 5 8)经t′检验P <0 .0 1,均有显著差异 ,抑瘤率为 84.85 6 %。病毒分离实验只有肿瘤组织中分离到病毒。ELISA实验实验组阳性。光镜和电镜下可见肿瘤细胞的坏死与凋亡及炎性细胞浸润。结论 新城疫病毒治疗人喉癌裸鼠模型有效 ,不侵犯正常组织。
Objective To explore the biological therapy of human laryngocarcinoma. Methods Nude mice model bearing laryngocarcinoma were established using human laryngeal squamous carcinoma cell line (Hep II). A large of newcastle disease virus(NDV) were injected into the tumor. The microstructural and ultra micro structural changes of carcinoma after administration of NDV were oberserved under light and electron microscopes. NDV was isolated in all tissue. The serums were examined by the method of ELISA.Results The difference was statistically significant, the average weight of the mice: t ′=2.397 P <0.05 , the average weight of the tumor tissue: t ′=2.852 P <0.05 , the average volume of the tumor tissue: t ′=6.058 P <0.01 . The NDV was isolated in only the tumor tissue of the experimental group .The experimental group was positive in ELISA. The necrosis and apoptosis of the tumor cell and inflammatory cellular infiltration were found under light and electron microscope. Conclusion These studies show that NDV is useful for laryngocarcinoma, that NDV is not encroach on normal tissue.
出处
《哈尔滨医科大学学报》
CAS
2001年第3期164-166,F003,共4页
Journal of Harbin Medical University
基金
黑龙江省自然科学基金资助项目 ( 96 0 5 )
关键词
喉肿瘤
裸鼠
新城疫病毒
生物学治疗
laryngocarcinoma
mice,nude
newcastle disease virus
biological therapy
