摘要
目的:研究新城疫病毒(NDV)对鼻咽癌细胞的抑制作用及其作用机制。方法:采用化学发光法、流式细胞术及电子显微镜研究NDV分别作用于两株鼻咽癌细胞后其对细胞生长的抑制情况,以及NDV诱导鼻咽癌细胞发生凋亡的情况。结果:化学发光法的结果显示,在一定的浓度范围和时间范围内,NDV对鼻咽癌细胞的抑制作用有剂量效应比和时间效应比关系。流式细胞术结果显示,NDV使鼻咽癌细胞生长阻滞在G1期,并促进细胞凋亡。电子显微镜观察细胞凋亡超微结构的结果显示,NDV可诱导鼻咽癌细胞产生凋亡小体,发生凋亡。结论:NDV可以抑制鼻咽癌细胞增殖,其作用机制主要是影响细胞生长周期和诱导细胞发生凋亡。
OBJECTIVE：To find out the role of newcastle disease virus（NDV） curing the NPC by using the technique of the cell biology and the molecular biology. METHODS：ATP method,flow cytometer （FCM） method,plaque formation and electronic microscope method were used to detect the inhibition effect of NDV on NPC and the effect on NPC appotosis. RESULTS：ATP method showed,NDV could inhibit the growth of NPC cells and the inhibition was depend on amount and time in the certain consistency and time. FCM method showed,NDV could make NPC cells stop in the period of G1 and lead to apoptosis. Electronic microscope method showed,NDV could induce apoptosis of NPC cells. CONCLUSION：It manifest that NDV may both inhibit the growth of NPC cell,and they can cause apoptosis of NPC cells.
出处
《中华肿瘤防治杂志》
CAS
2010年第1期20-23,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
广西科学基金项目(桂科自0640160)
关键词
鼻咽肿瘤
新城疫病毒
凋亡
细胞周期
nasopharyngeal neoplasms
newcastle disease virus
apoptosis
cell cycle
