摘要
采用LC/(-)ESI-MS法对银杏叶中的致敏性成分银杏酚酸进行了鉴定.利用次级化学平衡理论,建立了银杏酚酸的反相银化高效液相色谱分离方法.从色谱热力学角度对银离子分离银杏酚酸的影响进行了考察.在流动相中添加银离子,降低了具不饱和侧链银杏酚酸的保留时间,且银离子浓度与容量因子的对数之间存在良好线性关系.在确定的色谱条件下,四种银杏酚酸均获得较高的分离度,表明反相银化液相色谱是分离碳数与饱和度不相同的银杏酚酸同系物的有效方法.
Liquid chromatography - electrospray ionization mass spectrometry (LC/( -)ESI - MS) has been applied to identify Ginkgolic acids, which are allergenic components existing in Ginkgo bilobal leaves. The complete baseline separation of four Ginkgolic acids by reversed phase argentation HPLC has been firstly achieved. Effects of the silver ion content in mobile phase and different temperature on the separation of Ginkgolic acids were studied. Addition of silver ions into the mobile phase decreased retention of Ginkgolic acids with an unsaturated side chain. A linear relation between Igk' and [Ag+ ] was found. It also has been observed that temperature has great effect on selectivity of argentation HPLC. A possible explanation on the thermodynamic parameters is given. Resolutions between four Ginkgolic acids were above 1.5 under the optimum chromatographic conditions. The high selectivity of reversed phase argentation HPLC suggested that secondary chemical equilibrium in HPLC was an excellent method for separation of Ginkgolic acids homologous with different carbon atoms number and saturation.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2001年第7期1153-1157,共5页
Acta Chimica Sinica
