摘要
合成了一种新的吸附偶联试剂-硫酚乙酸琥珀酰亚胺酯(TPD),将TPD吸附在金的表面,利用其琥珀酰亚胺基与客体氨基的反应,将单链DNA修饰在金电极上作为探针,以电化学方法检测能与之互补DNA样品的碱基序列和含量.杂交后的DNA采用自行合成的双[1-二茂铁氨甲酰丙基-四氢化吡嗪-4-丙基氨甲酰吡啶]并吩嗪(FCZ)作指示剂进行检测,该化合物能够嵌入DNA双螺旋结构的碱基对中,显示出高度电化学活性.该传感器的电流信号与DNA浓度在2×10-3～1×100-7 mol/L范围有线性关系,检测限为8×10-10 mol/L.本方法可用于检测爱滋病和乙肝病毒DNA特征序列的研究.
A new modifier N - [6 - (thien - 3 - yl)acetoxy] - pyrrolidine - 2,5 - dione(TPD) and a sensitive electroactive indicator di[1 - (ferrocene - carbamoylpropyl) - tetrahydropyrazine - 4 - (propyl carbamoyl - pyridine) -] phenazine (FCZ) have been synthesized. TPD is adsorbed on gold surface to immobilize ssDNA as probe. FCZ is intercalated in dsDNA and detected by differential pulse voltammetry. The biosensor is based on a chemically modified electrode, on which TPD - ssDNA is self - assembled. It is sensitive to cDNA with the detection limit being 8 x 10(-10) mol/L. The current signal of the biosensor is proportional to the concentration of cDNA in the range of 2 x 10(-8) to 1 x 10(-7) mol/L. This electrochemical biosensor has been used to detect sequence - specific DNAs related to HIV - 1 and HBV virus.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2001年第7期1125-1129,共5页
Acta Chimica Sinica
基金
国家自然科学基金，浙江省自然科学基金
