摘要
目的 探讨鼻咽癌染色体脆性部位FRA3B区域的微卫星不稳定性 (MicrosatelliteInstability ,MSI)。方法 选择FRA3B区域附近的 6个微卫星多态标记对 30例鼻咽癌进行MSI分析。结果 MSI的发生率为6 3.33% (19/ 30 ) ,其中复制错误 (ReplicationErrors ,RER)阳性率为36 .6 7% (11/ 30 )。MSI频率较高的 3个位点为D3S15 47(30 .8% )、D3S1313(34 .6 % )和D3S130 0 (37.5 % )。临床Ⅰ～Ⅱ期患者MSI频率高于Ⅲ～Ⅳ期 (P <0 .0 5 )。结论 揭示FRA3B脆性部位的MSI为鼻咽癌形成过程中的早期分子事件 。
Objective To investigate the microsatellite instability(MSI) near the fragile site FRA3B in nasopharyngeal carcinoma (NPC). Methods Six microsatellite polymorphic markers around FRA3B were selected to detect MSI in 30 cases of NPC. Results The total frequency of MSI in all the 30 NPC was 63.33% (19/30) with a higher rate at loci D3S1547 ( 30.8% ), D3S1313 ( 34.6% ) and D3S1300 ( 37.5% ). Positive rate of replication errors (RER) rate was 36.67%(11/30). The frequencies of MSI in tumors staged Ⅰ～Ⅱ were significantly (P<0.05) higher than tumors those of staged Ⅲ～Ⅳ. Conclusion MSI may be a relatively early molecular event and may participate in nasopharyngeal carcinogenesis.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2001年第2期98-100,共3页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
