摘要
NOR1是与鼻咽癌密切相关的抑瘤/易感基因候选者之一,将NOR1基因的全长cDNA片段亚克隆至pcDNA3.1(+)的表达载体中,通过脂质体介导转染入鼻咽癌细胞系HNE1,RT-PCR、RNA印迹方法筛选高效表达NOR1的细胞株,并借助细胞生长曲线、软琼脂生长集落形成大小试验、流式细胞仪对转染细胞的生物学行为进行检测.结果发现转染了NOR1基因的HNE1细胞生长速度明显减慢,在软琼脂中的集落形成较对照组明显减小.流式细胞仪分析表明,NOR1可以延缓细胞由G0/G1期进入S期.结果表明NOR1基因可能在抑制鼻咽癌的发生发展中起重要作用,为进一步研究NOR1的功能研究打下基础.
NOR1 is one of the candidate tumor suppressor genes associated with nasopharyngeal carcinoma （NPC）. The mammal expression vector of NOR1, pcDNA3.1 （＋）/NOR1 was constructed and was introduced into HNE1 cell to explore the effect of NOR1 gene on HNE1. The integration of the exogenous vector DNA and the reexpression of NOR1 were detected by RT-PCR and Northern blot respectively. Finally, the cytobiological characterization of positive clone was analyzed by cell growth curves analysis, soft agar assay, cytometry. The growth of HNE1 cells transfected with NOR1 gene was dramatically inhibited compared with the parent HNE1 cells. Flow cytometric data showed that more NOR1 transfeeted cells went into G0/G1 phase than controls, and it also presented decreased clonogenicity in soft agar. The research indicated that the NOR1 could play a critical role in the progression of NPC due to its results providing a basis study of the function of it.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2005年第8期777-780,共4页
Progress In Biochemistry and Biophysics
基金
国家高技术"863"计划(2001AA221031)
国家自然科学基金(30300383)
中国博士后科学基金(2004035206)资助项目~~
关键词
鼻咽肿瘤
NOR1基因
细胞转染
nasopharyngeal carcinoma, NOR1 gene, cell transfection
