摘要
根据番茄环斑病毒 (TomRSV)外壳蛋白基因序列设计的引物P1,P2 ,用感病及健康组织总RNA为模板 ,进行RT PCR试验 ,结果从感病组织中扩增出了 470bp的目的片段 ,而健康组织中无此扩增带。摸索各项实验条件 ,建立了RT PCR检测番茄环斑病毒 (TomRSV)
A pairs of primers of P1,P2 were designed,corresponding TomRSV RNA 2. The cDNA was made,and PCR was gotten on,with total RNA of the infected tissue and health tissue.The target fragment about 470bp was amplified from the infected sample, but not from health sample.More condition were tested, The method of detection of TomRSV by RT-PCR was built.
出处
《云南农业大学学报》
CAS
CSCD
2001年第2期96-98,共3页
Journal of Yunnan Agricultural University
基金
云南省院省校合作研究项目
