摘要
目的 探索散发性大肠腺瘤细胞的原代培养 ;探讨环氧合酶 (COX) 2选择性抑制剂对大肠肿瘤防治的作用机制。方法 从人大肠腺瘤中分离培养上皮细胞 ;检测COX 2选择性抑制剂NS 398对细胞增殖的影响和大肠肿瘤中COX 2蛋白的表达。结果 取得大肠腺瘤 30枚 ,培养成功 2 3枚 ;NS 398对腺瘤细胞的抑制作用具有时间及剂量依存性 ;在大肠肿瘤中 ,COX 2蛋白表达显著增加 ,分别为 83 1%和 80 0 %。结论 避免污染、恰当的消化时间、合适的细胞数和成纤维细胞的去除 ,是大肠腺瘤细胞原代培养成功的关键 ;NS 398对细胞生长具有明显抑制作用 ;COX
Objective To investigate the mechanism of COX 2′s selective inhibitor, NS 398, on human colorectal adenoma development. Methods The human colorectal adenoma cells were isolated and cultured identified. The cell growth rates were assessed ,when treated with NS 398 ,by using MTT method. Expression of COX 2 protein in human colorectal tumor was examined by immunohistochemistry. Results 23 of 30 colorectal adenomas were primary cultured successfully. NS 398 inhibited the proliferation of the cells in a dose and time dependent manner. COX 2 was overexpressed in colorectal adenoma(83.1%) and cancer(80.0%)compared with normal colon mucosa. Conclusion The key of the several culture conditions for the survival of human colorectal adenoma cells have been improved. Such as, best of the digestive time and number of cell. The cells can be inhibited by NS 398 with dose dependent. Overexpression of COX 2 protein occurs early during colorectal carcinogenesis which contribute to tumour formation.
出处
《中华内科杂志》
CAS
CSCD
北大核心
2001年第6期377-380,W001,共5页
Chinese Journal of Internal Medicine
