摘要
目的:了解骨关节炎(OA)滑膜和关节软骨中金属蛋白酶组织抑制物-1(TIMP-1)的分布及变化,探讨OA滑膜和关节软骨中TIMP-1变化与OA发病和治疗的关系。方法:应用免疫组织化学方法对26例OA及3例正常人的滑膜和关节软骨中TIMP-1的分布和阳性程度进行观察。结果:17例OA滑膜标本TIMP-1染色阳性,分布于滑膜衬里细胞、血管内皮细胞和间质巨噬细胞等部位。阳性颗粒位于细胞浆中,程度多为弱阳性。16例OA关节软骨标本TIMP-1染色阳性。讨论:OA患者滑膜和关节软骨中TIMP-1分布较少和含量较低可能与OA的发病有一定关系。设想利用TIMP-1或其类似物可抑制关节软骨破坏和改善OA的病情。
To study the expression of tissue inhibitor of matalloproteinase- 1 (TIMP- 1) in the synovium and articular cartilage of patients with osteoarthritis (OA) and to explore the possible relationship between TIMP - 1 and pathogenesis and treatment of OA. Methods: The distribution and positive degree of TIMP- 1 in the synovium and the articular cartilage of 26 patients with OA and 3 traumatic patients whose joints were not injuried were studied with an immunohistoche-mical method. Results: The immunohistochemical staining of TIMP- 1 showed positive in 17 synovial samples of 26 patients with OA. The positive staining of TIMP- 1 was most found in synovial lining cells, and sometimes found in endothe-liocytes and macrophages in subsynovial layer. The positive particles were distributed in the cytoplasma of these cells and the staining levels of TIMP - 1 were weak in the synovium of patients with OA. The immunohistochemical staining of TIMP -1 showed positive in 16 samples of articular cartilage in 26 patients with OA. The positive staining of TIMP - 1 was found in the cytoplasma of chondrocytes. Conclusion: There was a possible relationship between the less level and distribution of TIMP- 1 in the synovium and articular cartilage of patients with OA and pathogenesis of OA. It may be assumed using exogenetic TIMP - 1 and its analogues to inhibit cartilage damages and to improve the pathologic change of OA.
出处
《天津医药》
CAS
北大核心
2001年第9期529-531,F002,共4页
Tianjin Medical Journal
关键词
金属蛋白酶类组织抑制剂
骨关节炎
滑膜
关节软骨
tissue inhibitor of metalloproteinases osteoarthritis synovial membrane cartilage, articular
