摘要
目的 探讨骨关节炎 (OA)患者关节软骨和滑膜中转化生长因子 β1(TGFβ1)、转化生长因子 β受体 (TGFβR)和金属蛋白酶组织抑制物 1(TIMP 1)变化及其与OA发病的关系。 方法 采用免疫组化方法检测 2 3例老年OA患者及 3例外伤患者正常关节软骨和滑膜TGFβ1、转化生长因子 βⅠ类受体 (TGFβRⅠ )、转化生长因子 βⅡ类受体 (TGFβRⅡ )和TIMP 1的分布和阳性程度。 结果 OA患者中 14例关节软骨和 16例滑膜TGFβ1染色呈阳性或弱阳性 ,14例关节软骨和 15例滑膜TIMP 1染色呈弱阳性 ,全部OA患者关节软骨和滑膜TGFβRⅠ和TGFβRⅡ染色呈强阳性。阳性细胞包括软骨细胞、滑膜衬里细胞、滑膜下层的血管内皮细胞和间质巨噬细胞等。 结论 老年OA患者关节软骨和滑膜中TGFβ1、TGFβR及TIMP
Objective To explore the changes of transforming growth factor beta 1(TGFβ 1), TGFβ receptor(TGFβR), and tissue inhibitor of metalloproteinase 1(TIMP 1), as well as their relations to the pathogenesis of elderly osteoarthritis. Methods The distribution and the levels of TGFβ 1, TGFβ receptor Ⅰ(TGFβRⅠ) , TGFβ receptor Ⅱ(TGFβRⅡ) and TIMP 1 in the articular cartilage and the synovium of 23 aged patients with osteoarthritis and 3 patients with trauma(the joints were not injured) were examined using immunohistochemical methods. Results The immunohistochemical staining for TGFβ 1 showed positive or weakly positive in the articular cartilage and the synovium in two thirds of the patients with osteoarthritis. The strongly positive staining for TGFβRⅠ and TGFβRⅡ was found in the articular cartilage and the synovium in all of the osteoarthritis patients. The immunohistochemical staining of TIMP 1 showed weakly positive in the articular cartilage and the synovium of two thirds of patients with osteoarthritis. The positively stained cells containing TGFβ 1, TGFβRⅠ, TGFβRⅡ and TIMP 1 included chondrocytes, synovial lining cells, endothelial cells and the macrophages in subsynovial layer with osteoarthritis. Conclusions The distribution and the content of TGFβ 1, TGFβR and TIMP 1 in the articular cartilage and the synovium of osteoarthritis in the elderly are considered to correlate with the pathogenesis of osteoarthritis.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2001年第2期113-116,共4页
Chinese Journal of Geriatrics
