摘要
为探讨一氧化氮 (NO)、肿瘤坏死因子 - α(TNF- α)、白细胞介素 - 6 (IL- 6 )、白细胞介素 - 12 (IL- 12 )在重型肝炎 (SH)并发自发性细菌性腹膜炎 (SBP)患者血清和腹水中的水平及其临床意义 ,分别采用 Griess比色法及放射免疫法 (RIA)检测了 45例 SH并发腹水患者血清及腹水中 NO、TNF- α、IL- 6和 IL- 12含量 ,其中腹水感染组 30例、腹水非感染组 15例 ,结果显示 SH并发 SBP血清和腹水 NO、 TNF- α、 IL- 6和 IL- 12水平均明显高于腹水非感染组 (P<0 .0 5 ) ,其升高水平与血清总胆红素 (SB)、凝血酶原时间 (PT)呈正相关 ,与血清胆固醇呈负相关 (P<0 .0 1)。感染控制后血清和腹水 NO、 TNF- α、 IL- 6、IL- 12水平均明显下降 (P<0 。0 5 ) ,但仅能降至非感染组水平。表明 :NO、 TNF-α、IL- 6和 IL- 12参与 SH及 SBP形成腹水的发生、发展过程 ,其联合检测对 SH并发 SBP的早期诊断。
The possible immunopathogenic role of NO, TNF α, IL 6 and IL 12 in the patients with severe viral hepatitis (SH) complicated by spontaneous bacterial peritonitis (SBP) and their significance were investigated. The levels of NO, TNF α, IL 6 and IL 12 in sera and ascites were detected by radioimmnoassoy (RIA) and Griess respectively in 45 patients with SH complicated by ascites, including 30 cases of SH complicated by SBH and 15 cases of SH without SBP. The levels of NO, TNF α, IL 6 and IL 12 in sera and ascites in the patients with SH complicated by SBP were significantly higher than those without SBP ( P <0 05), and the elevated levels of NO, TNF α, IL 6 and IL 12 were positively correlated with serum bilirubin (SB), prothrombin time (PT) and negatively with total cholesterol (T ch) ( P <0 01). When infections were controlled, the levels of NO, TNF α, IL 6 and IL 12 in sera and ascites were significantly decreased ( P <0.05), but only to the levels as those in the patients without SBP ( P <0.05). It was indicated that the abnormal elevation of NO, TNF α, IL 6 and IL 12 in serum and ascites may dead to immunopathogenic reaction resulting in necrosis damage and endotoxemia. The combined detection of NO, TNF α , IL 6 and IL 12 might be helpful for the early diagnosis of abdominal cavity infection and the evaluation of the disease severity and prognosis.
出处
《同济医科大学学报》
CAS
CSCD
北大核心
2001年第4期368-368,370,共2页
Acta Universitatis Medicinae Tongji
