摘要
对氢化碘硝基四唑 (INT)用于大鼠及人体皮肤组织活力测定的可行性及方法学进行了研究。分组测定了不同 INT浓度、 EDTA浓度、浸出剂、浸出时间对检测结果的影响 ,对反应条件进行了优化。证实本法可在有氧条件下 ,反应与浸出同时进行 ,操作简便 ,时间较短 ,灵敏度较高 ,所需皮片量少 。
The feasibility and the methodology of the measurement of the skin viability of SD rats and men were studied with indonitrotetrazolium chloride (INT). Grouping experiments were done under varied concentrations of INT and EDTA, different lixiviums and varied time of reaction and lixiviation. In this method reaction and lixiviation can be going the same time without the need of dispersing the oxygen. This method is fit for the clinical application for its simple operation, short time, high sensitivity and less amount of needed skin flap.
出处
《同济医科大学学报》
CSCD
北大核心
2001年第4期310-312,共3页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金资助项目 (No. 39870 2 0 6 )
