摘要
目的 研究一种利用伏安型生物传感器检测 (计数 )微生物细胞 (啤酒酵母菌 )的新方法。方法 该传感器由一个平面热解石墨电极、铂电极及Ag/AgCl电极的三电极系统组成。将阻留啤酒酵母菌细胞的滤膜紧附在工作电极表面 ,然后在工作电极与对极间施加一扫描电压 ,进行半微分循环伏安扫描 ,记录伏安图谱 ,依据峰电流计数微生物。结果 本实验选用啤酒酵母菌为实验菌种 ,探讨了啤酒酵母菌浓度与峰电流的关系。发现膜上细胞数在 5× 10 6 ～ 2 5× 10 7范围内与峰电流呈线性关系。相关系数为 0 990 1,直线回归方程为Y =0 66+0 5 6X(× 10 7) ,检测下限为 2 8× 10 6 cells。结论 提示我们可利用峰电流进行微生物活细胞的快速计数。
Objective To study the method of the detection of microbial cell by a voltammetric Biosensor.Method An electrochemical method for detection of microbial cells was developed by appling semi-differential cyclic voltammetry to microbial cells.A modified basal plane pyrolytic graphite electrod was empolyed as a working electrod.Applying a sweep voltage between a working electrod and a counter electrod while the microbial cells on a membrane filter were brought into contact with the working electrod.Then measure the generated current between the electrodes in phosphate buffered soline.Results The anodic peak potential of S.cerevisiae was 0.75V(vs Ag/AgCl electrod).The relationship between the peak current and the cell concentration of S.Cerevisiae was studied.The anodic peak current was proportional to the cell numbers on the membrane filter in a range of (0.5-2.5)×10 7 cells.Regression equation Y=0.66+0.56X(×10 7),correlation coefficient 0.9901.The minimum detectable cell number was 2.8×10 6 cells.Conclusion Cell numbers of microbial cells could be determined from the anodic peak current of semi-differential cyclic voltammetry.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2001年第9期849-850,共2页
Chinese Journal of Public Health
