摘要
目的 观察维生素A的胚胎毒性和致畸作用 ,以确定VA 的致畸剂量。方法 在小鼠胚胎器官形成期 ,经口给予孕鼠维生素A(VA) 1 5 g/kg、2 0 g/kg、2 7g/kg ,于孕后第 18天剖腹取仔 ,观察其活胎外观有无畸形 ,并对胎仔、骨骼及内脏进行检查。以生理盐水作为对照组。结果 高剂量组交配成功率明显下降 ,第 18天处死前孕鼠体重无明显变化 ,但中剂量组母鼠增重明显减少 (P <0 0 5 ) ,高剂量组母鼠增重高于中剂量组 ,低于阴性对照组。仔鼠统计指标中 ,吸收胎率、死胎率显著高于阴性对照组 (P <0 0 5 )。各剂量组间吸收胎率也呈显著变化 (P <0 0 5 )。VA3个剂量组的活胎外观畸形率依次增高 ,且高剂量组与阴性对照组比有显著性 (P <0 0 5 ) ,各组的骨骼畸形率均显著高于阴性对照组 (P <0 0 5 ) ,并且各组间也有显著变化 (P <0 0 5 )。内脏检查末见畸形。结论 VA 作为孕鼠致畸实验的阳性对照品 ,以经口给予 2mg为宜。
Objective The teratogenetic effect of three different dosages of vitamin A in mice was observed in order to ascertain the teratogenetic dose of vigtamin A.Motheds At the phase of organ formation of embryo,pregnant mice were treated orally with 1.5,2.0,2.7 g/kg vitamin A.At the 18th day of pregnant,embryo were brought out by cesarean section.Outlook,bone and internal organs of live embryo were checked for malformation.Normal saline was used as control group.Results Rate of absorbed embryo and died embryo in all vitamin A treated groups was significantly higher than that in control group (P<0.05).Malformation rate of live embryo outlook in 2.7g/kg group increased obviously,malformation rate of bone in all vitamin A treated groups increased significantly compared with control group (P<0.05).No internal organ malformation was observed.Conclusion The proper dosage of vitamin A,as a positive control in teratogenetic test,should be 2g/kg.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2001年第9期809-810,共2页
Chinese Journal of Public Health
关键词
维生素A
剂量
小鼠
致畸胎
vitamin A
dose
mouse
teratogenetic effect
