摘要
用反转录套式PCR(RT -PCR)法从陕西地区 1例NANB型肝炎患者血清中扩增出部分E区的cDNA片段 ,克隆于PGEM -T载体中用全自动序列分析仪进行双向测序 ,测得的序列以及已发表的同一分离株 5′NRC、C区序列 ,分别同Genbank中注册的 3 6株已知基因型的HCV相应区段的序列比较 ,构建种系发生树。结果 5′NCR区相应序列构建的种系发生树表明该分离株为 2型 ,而用C区和E区构建的种系发生树均表明该分离株为 2a型。提示该分离株为 2a型 ;对同一病原体来说 ,其基因区段的变异度不同 ,则种系发生树分析的结果不完全相同 ,变异度大的结果较准确。
Objective To clone and sequence partial cDNA of Hepatitis C virus(HCV) genome of Shanxi China,constructed phylogenetic tree.Methods A 878bp fragment in envelope region was amplified by reverse transcription nested polymerize chain reaction (RT-PCR).The fragment was cloned into PGEM T-vector,then sequenced by auto-sequencing instrument.Phylogenetic tree were constructed from the 5′NCR sequene of 252bp,C sequence of 436bp and E sequence of 878bp by HCV-XA and other 36 strains registered in Genbank respectively.Results Phylogenetic tree constructed from the 5' NCR indicates HCV-XA belonged to type 2;from C and E indicate it belonged to subtype 2a.Conclusion The isolated strain belonged to type 2a.The variation of the subgenome affected the result of phylogenetic analyses for the same pathogen.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2001年第9期769-771,共3页
Chinese Journal of Public Health
