摘要
目的研究双氢青蒿素治疗对卡氏肺孢子虫肺炎大鼠血清和肺泡巨噬细胞培养上清液 TNF- α水平的影响。方法以醋酸可的松皮下注射 Wistar大鼠建立卡氏肺孢子虫肺炎动物模型 ,用 60 m g/ kg双氢青蒿素治疗实验大鼠 ,杀鼠取肺 ,用胶原酶消化法分离肺泡巨噬细胞 ,用 L PS刺激培养 72 h,同时设有感染组和正常对照。用 TNF- α试剂盒分别检测血清和培养上清液 TNF- α的水平。结果感染组和治疗组 TNF- α水平均高于正常对照 ,治疗组 TNF- α水平则低于感染组。结论卡氏肺孢子虫感染引起大鼠肺泡巨噬细胞分泌高水平的 TNF- α,但双氢青蒿素治疗后 PCP大鼠肺泡巨噬细胞产生 TNF- α水平降低。
ObjectiveTo study the effects of treatment with dihydroartemisinin(DHA) on TNF-α in sera and the supernatant of alveolar macrophages(AMs) in rats infected with Pneumocystis carinii pneumonia(PCP). Methods Wistar rat model of PCP was established by subcutaneous injection with cortisone acetate. Experimental rats were treated by 60 mg/kg DHA and killed to obtain lungs;AMs were separated by collagenase digestion and cultured by LPS stimulation in 72 hours. Infected and normal rats served as controls,the levels of TNF-α in sera and the supernatant of AMs were detected by TNF-α kits res- pectively. Results The levels of TNF-α in the infection group and treatment group were higher than that in the normal control respectively, but those in the treatment group were lower than that in the infection group. Conclusion AMs of PCP rats produce high level of TNF-α,but the level of TNF-α decreases after DHA therapy.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第4期274-276,294,共4页
Immunological Journal
基金
国家自然科学基金资助项目 ( 3 9670 65 8)
