摘要
目的 研究比色法测定复方虫草颗粒中甘露醇的含量 ,建立一种准确、可行的含量测定方法。方法 在供试品中依次分别加入氧化剂高碘酸钾、L-鼠李糖与 Nash试剂 ,5 3℃水浴加热 15 m in使呈色 ,在 412 nm处测定吸光度 ,用比色法测定其中甘露醇的含量。结果 在 10～ 5 0μg/ m L范围内对 3种不同批号样品在实验条件下测定 ,其平均含量为 1.2 7% ,本法平均回收率为 10 0 .3% ,RSD为 0 .83% (n=5 )。结论 可作为复方虫草颗粒中甘露醇含量测定及有关质量控制的一种简便、准确和快速的方法。
Object To develop a practical method for the assay of mannitol in FUFANG CHONGCAO KELI (FCK) by colorimetry Methods Content of mannitol in the granule was assayed colorimetrically by determining its absorbance at 412 nm, after sequential addition of potassium periodate, L rhamnose and Nash reagent to sample solutions and warmed at a bath temperature of 53 ℃ for 15 min Results Samples from three different batches of FCK were analyzed according to the above analytical condition The average mannitol content in FCK was found to be 1 27%, when the contents of reference standard mannitol in solution were in the range of 10 μg to 50 μg/mL, the mean recovery was 100 3% and RSD=0 83% (n= 5) Conclusion The method may be used as a simple, accurate and rapid way to determine the content of mannitol in FCK as well as the quality control for the product
出处
《中草药》
CAS
CSCD
北大核心
2001年第8期697-699,共3页
Chinese Traditional and Herbal Drugs
关键词
复方虫草颗粒
甘露醇
比色法
FUFANG CHONGCAO KELI (compound cordyceps granule) (FCK)
mannitol
co lorimetry
