摘要
目的 探讨线粒体钙浓度 (MCC)、自由基和胞内酶释出之间的相互关系以及细胞内钙浓度升高的来源。方法 通过不同Ca2 +浓度下大鼠离体骨骼肌被动牵拉培养的模型 ,测定MCC、丙二醛 (MDA)浓度和肌酸激酶 (CK)活性。结果 MCC在 3mmol/LCa2 +培养液中培养 2、4h ,在 3mmol/LCa2 +与无Ca2 +培养液中均牵拉培养 2h ,各实验中均有显著性增加 (P <0 0 5 ) ,而在无Ca2 +培养液中 2h培养无显著性变化 (P >0 0 5 ) ;CK在 3mmol/LCa2 +与无Ca2 +培养液中均牵拉培养 2h实验中有显著增加 (P <0 .0 5 ) ,其余各实验无明显变化 (P >0 0 5 ) ;MDA在各实验均无显著变化 (P >0 0 5 )。结论 MCC在牵拉负荷条件下有显著增加 ,细胞外Ca2 +浓度在胞内Ca2 +增多中起着重要作用 ,胞外Ca2 +流入是胞内Ca2 +浓度增高的主要来源 ;CK释出似乎与牵拉负荷有关 ,且与细胞外 (培养液中 )Ca2 +浓度有一定联系 ;胞内钙平衡紊乱可能发生在脂质过氧化之前 。
Objective To study the relationship among muscle mitochondrial [Ca 2+ ](MCC), free radicals and the efflux of cytoenzyme, as well as the source of the elevated intracellular [Ca 2+ ].Methods Through the passive stretched model of rat isolated soleus in different [Ca 2+ ], the changes of MCC, malondialdehyde (MDA) and creatine kinase(CK) were examined.Results MCC appeared to be elevated significantly during incubation in 3 mmol/L Ca 2+ buffer for 2 hours, 4 hours, in 3 mmol/L Ca 2+ and Ca 2+ free buffer for 2 hours all under stretch conditions ( P <0.05), but no significant change in Ca 2+ free buffer for 2 hours was shown. CK were significantly increased in 3 mmol/L Ca 2+ and Ca 2+ free buffer for 2 hours all under stretch conditions ( P <0 05), but no significant changes of CK was shown in other experiments (P >0 05). MDA had no significant changes in all experiments ( P >0 05). Conclusion MCC was shown to be elevated significantly in the condition of stretch load, the concentration of external calcium might play an important role in the elevation of intracellular [Ca 2+ ], the influx of Ca 2+ from the external might be the central source of the elevated intracellular [Ca 2+ ]. The release of CK seemed to be related with the stretch load and the intracellular [Ca 2+ ]. Disturbances of the intracellular calcium hemeostasis might occur before lipid peroxidation, and might be the initial factor of muscle injury.
出处
《中国职业医学》
CAS
北大核心
2001年第3期8-10,共3页
China Occupational Medicine
