摘要
目的 : 阐明克山病心肌细胞损害的电生理机制。方法 : 用细胞内微电极技术分别观察正常对照、单纯低 Se、低 Se高维生素 C、单纯低 Cr、低 Cr高维生素 C、低 Se低 Cr、低 Se低Cr伴高维生素 C饲料饲养 1 4w后大鼠心肌细胞电生理的改变。结果 : 单纯低 Cr组无变化 ;单纯低硒组静息电位 ( RP)、动作电位幅度 ( APA)降低、动作电位时程 ( APD50 、APD90 )延长 ;低 Se低Cr组 RP、APA降低 ,APD50 、APD90 明显延长 ;低 Se高维生素 C组仅 RP降低 ,APA、APD与对照组相比差异无显著性意义。结论 : 低 Se低 Cr可明显改变心肌细胞的电生理特性 ,维生素 C可以对抗低 Se低 Cr所致的这种变化。
Objective:To elucidate the etiology of the impairment of myocardial cells in Keshan disease. Methods:With intracellular microelectrode technique, we observed the electrophysiological changes of the eight groups of rats fed separately:normal, low Se, low Se+high VC, low Cr, low Cr+high VC, low Se+low Cr and low Se+low Cr+high VC fodders. Results:Compared with the control, there was no change in low Cr group. However, in low Se group, RP and APA decreased, APD 50 and APD 90 prolonged. In low Se+low Cr group, APA decreased, APD 50 and APD 90 prolonged significantly. In low Se with high vitamin C groups, though RP still decreased, no change had been detected on APA and APD. Conclusion:It is suggested that Se and Cr deficiency may jointly change the electrophysiological properties of myocardial cells significantly. However, vitamin C may protect myocardial cells against the injury effectively.
出处
《营养学报》
CAS
CSCD
北大核心
2001年第2期114-116,共3页
Acta Nutrimenta Sinica
基金
山东省教委基金!( No.J97K10 )
