摘要
目的 探讨参附注射液 (SF)对休克复苏期间肠黏膜损伤的保护作用机制。方法 通过兔失血性休克复苏模型 ,2 1只家兔随机分为参附注射液治疗组 (A组 )、单纯复苏组 (B组 )和假手术组 (C组 ) ;A组于复苏同时首剂静注参附注射液2 .1ml/kg ,随后静滴 5ml/kg ,直到观察结束。分别于实验前 (S0 )、休克 1h(S1)及复苏 1h(R1)、3h(R3)观察乙状结肠黏膜内pH(pHi)、肠黏膜NO、MDA及钙 (Ca++)含量。结果 A组R1和R3时肠pHi明显高于B组相应值 (P <0 .0 5 ) ,R3时肠黏膜NO、MDA及Ca++含量低于B组 (均P <0 .0 5 ) ;B组复苏期间肠pHi维持在S1时的低水平状态 ,肠黏膜NO、MDA及Ca++含量均明显高于A组和C组 (P <0 .0 5 )。结论 SF对失血性休克复苏期间肠黏膜保护作用的机制可能为增加肠黏膜灌注及氧合、抑制NO的产生及抗炎效应。
Objective To investigate the mechanism of protective effect of shenfu(SF) on imtestinal mucosa during hemorrhagic shock-reperfusion in rabbits.Methods Using the rabbit model of hemorrhagic shock-reperfusion,twenty-one rabbits were divided randomly into 3 groups:SF groups(A),Shock-reperfusion Group(B)and control group(C).Intestinal intramucosal pH(pHi)of sigmoid colon,nitric oxide(NO),malondialdehyde(MDA)and Ca ++ contents of intestinal mucosa were measured before shock(S 0 ),1 hour after shock(S 1 ),1 hour(R 1 )and 3 hour(R 3 )of reperfusion.Results pHi in group A were significantly higher than those in group B during reperfusion.NO,MDA and Ca ++ contents of intestinal mucosa were significantly lower at R 3 than those in group B respectively ( P <0.05).Compared with those in group C,NO and MDA contents of intestinal mucosa remained unchanged,but Ca ++ contents of intestinal mucosa increased significantly in group A at R 3 .Conclusions SF can protect intestinal mucosa from ischemia-reperfusion injury during hemorrhagic shock through improving perfusion and oxygenation,inhibiting the production of NO and anti-inflammation action,reducing oxide free radical and calcium overload. [
出处
《中国急救医学》
CAS
CSCD
北大核心
2001年第8期447-448,共2页
Chinese Journal of Critical Care Medicine
关键词
肠黏膜
再灌损伤
失血性休克
参附注射液
家兔
Intestinal mucosa
Reperfusion injury
Shock,hemorrhagic
Shenfu injection
