摘要
目的 观察维奥欣(VIOCIN)对动脉粥样硬化形成以及心肌缺血的影响。方法 采用高胆固醇饮食喂养20只大鼠,随机分为对照组和试验组,每组10只,试验组于高胆固醇饮食喂养7天后添加VIOCIN(80mg/kg),两组于喂养高胆固醇饮食前和喂养后第30天和第60天测血清胆固醇(TC)、三酰甘油(TG)和血浆内皮素(ET-1)水平,并测定心电图。喂养第60天处死动物作病变血管及心肌光镜检查和电镜检查。结果对照组喂养第30天、60天TC、TG和ET-1较喂养前明显升高(P<0.01),试验组TC、TG和ET-1则无显著变化(P>0.05)。光学显微镜检查:对照组主动脉根部局部管腔形成典型的动脉粥样硬化斑块,平滑肌细胞排列紊乱;试验组主动脉根部管壁厚薄均匀一致,平滑肌细胞排列整齐,内膜未见粥样斑块。电镜检查:对照组心肌线粒体肿胀,线粒体嵴稀疏,内可见大量空泡,毛细血管内皮电子密度降低;试验组心肌线粒体未见上述改变,毛细血管内皮电子密度较高,偶见空泡变性。结论VIOCIN能降低TC、TG和ET-1,防治动脉粥样硬化和心肌缺血的发生。
Objective To observe the influence of Viocin on coronary artery atherosclerosis and myocardial ischemia. Methods 20 rats fed with high cholesterol food (HCF)for 60days were divided into 2 groups (control group 10 and study group 10) . From the 7th day after feeding with HCF Viocin were added into the foods of the rats in the study group. The total serum cholesterol (TO , triglyceride (TG) , plasma endothelin-1 (ET-l)and ECG before feedingwith HCF and the 30th day and 60th day after feeding with HCF in Two groups were studied. The 60th day after feeding with HCF,all animals were killed to study with microscope and electron microscope. Results The TC, TG.ET-1 level of control group in the 30th and 60th day were significantly increased compared with that before feeding with HCF(P<0. 01). The TC,TG,ET-1 Level of study group in the 30th and 60th day were not changed comparing with before feeding with HCF(.P>0. 05). Light micrographs of aorta and coronary arteries in control group show the atherosclerosis plaque forming in the intima of aorta and inregular growth and proliferation of smooth muscle cells in the wall and narrowing or occlusion of the lumen, and the same changes were not seen in the study group. The scanning electron micrographs in control group demonstrate myocardial chondriosome swelling, thin chondriosome peak and a large number of empty bubble appearing inside it, capillary endothelial cell electron density reducing, while the same changes were not seen in the study qroup. conclusion Viocin could reduce TC.TG and ET-1 level, and prevent coronary artery atherosclerosis and narrowing.
出处
《中国心血管杂志》
2001年第4期196-199,共4页
Chinese Journal of Cardiovascular Medicine
