摘要
目的 探讨许旺细胞分泌相对分子质量为 67× 10 3蛋白对培养脊髓运动神经元营养作用。 方法 提纯许旺细胞分泌相对分子质量为 67× 10 3蛋白 ,取 SD胎鼠 (12～ 14d)脊髓腹侧运动神经元培养 ,鉴定 ,将脊髓运动神经元与不同浓度的相对分子质量为 67× 10 3 蛋白液联合培养 ,用 MTT法检测该蛋白生物活性。 结果 许旺细胞源性蛋白 (67× 10 3 )在不同的浓度下吸光度 (A)值不同 ,显示明显的量效关系 ,其最佳活性浓度为 2 0 ng/ ml。 结论 许旺细胞分泌的相对分子质量为 67× 10
Objective Exploration trophism of protein (relative molecular quantity 67×10 3) secreted by Schwann cell to motoneuron Methods Purfication of Schwann cell secretion neurotrophic protein (67×10 3) by method of Xiao Yuzhou Spinal cord ventral motoneuron (SCVMNs) of E 12～14 d (embryonic age 12～14 d) SD rats were harvested and cultured and identified SCVMNs were cultured in different concentration of protein (67×10 3) liquid Its neurobiological activity was assayed by MTT method Results The A values was not same in different concentration of protein (67×10 3) There was a dose effect relationship between biological effect intensity and protein dose, the optimum effect protein concentration of neurotrophic protein (67×10 3) was 20 ng/ml Conclusion The protein secreted by Schwann cell can maintain SCVMNs surval in culture
出处
《中华显微外科杂志》
CSCD
北大核心
2001年第3期189-191,共3页
Chinese Journal of Microsurgery
基金
美国中华医学基金会 (CMB)资助项目
