摘要
目的 :应用动态比浊法鲎试验定量测定盐酸川芎嗪注射液中的细菌内毒素含量。方法 :通过对样品中定量添加标准内毒素的干扰试验 ,检测其回收率应在 5 0 %～ 2 0 0 %范围。结果 :将样品进行 1/10 0稀释可以有效地消除其对鲎试验的干扰。结论 :EDS
Objective:The kinetic turbidimetric Limulus test was used in the endotoxin assay of Ligustrazin Hydrochloride injection. Methods: The validation test was fulfiled,when the amount of endotoxin spiked in Ligustrazin Hydrochloride injection sample was recovered in the range of 50%～200% .Results: A 100 fold dilution to Ligustrazin Hydrochloride injection was effective to eliminate the interference in Limulus test.Conclusion: The EDS 99 kinetic tube reader provides a new way for the detection of endotoxin in Ligustrazin Hydrochloride injection.
出处
《广东药学院学报》
CAS
2001年第2期93-95,共3页
Academic Journal of Guangdong College of Pharmacy
基金
广东省重点科技攻关项目! (NO 2KM 0 540 63)
关键词
动态比浊法鲎试验
盐酸川芎嗪
细菌内毒素
干扰试验
kinetic turbidimetric limulus test
ligustrazin hydrochloride
bacterial endotoxin
Inhibition enhancement test
