摘要
用NDLasota弱毒株、NDV地方分离株 (F基因Ⅶ型 )—HD1,分别接种鸡胚 ,收集尿囊液 ,制备病毒抗原液 ,福尔马林灭活后按一定比例混合 ,与白油佐剂乳化成油包水型乳剂疫苗 ,并对二价苗各项技术指标进行了测定 ,证明本疫苗安全、无副作用 ,接种后 3周产生免疫保护 ,攻毒保护率为 10 0 % ,接种 2周后通过免疫抗体检测 ,NDHI抗体效价达 8log2 .疫苗在鸡场应用 ,获得了满意效果 ,有效的控制了ND的发生与流行 .
Chick blastocysts were vaccinated with ND Lasota attenuated strain and NDV local isolated strain (F gene type Ⅶ) HD 1,respectively,followed by collection of allantoic fluid,preparation of viral antigen fluid,proportionate mixing after being killed with formalin,and emulsion into a oil coated water type emulsion vaccine with a white oil adjuvant.All technical parameters determined of the divalent vaccine showed that it was safe without side effects.Immunological protection happened with 100% challenging protection 3 weeks after vaccination.Examination of immunological antibodies 2 weeks after vaccination indicated that the antibody titer for NDHI reached 81og2.Application of this vaccine on some breeding chicken farms and laying hen farms has produced satisfactory results,effectively keeping Newcastle disease in check.
出处
《河南农业大学学报》
CAS
CSCD
2001年第2期182-184,共3页
Journal of Henan Agricultural University
关键词
鸡新城疫
二价疫苗
免疫
抗体
Newcastle disease
divalent vaccine
vaccination
antibody
