摘要
目的 探讨白细胞介素 6 (IL 6 )在新生儿败血症及败血症休克中的变化及临床意义。方法 采用酶联免疫吸附法(ELISA)检测正常与严重感染新生儿血清IL 6的水平 ,用逆转录聚合酶链反应 (RT PCR)技术测定外周血单个核细胞(PBMC)IL 6mRNA表达情况。结果 感染组血清IL 6水平升高 ,其中败血症性休克组尤为明显 ,并随病情好转而下降 ,检测时病程在 3d以内的患儿血清IL 6显著高于病程在 4d以上者 ;另检测 5例败血症患儿PBMC均见IL 6mRNA的表达 ,而 6例对照新生儿中仅 3例有IL 6mRNA表达。按百分位数法初步确定血清IL 6≥ 2 3ng/L为诊断新生儿败血症的参考指标 ,此指标敏感性为 81% ,特异性为 83 %。结论 新生儿败血症及败血症休克早期血清IL 6水平升高、IL 6mRNA表达增强 ,与病情、预后有关 ,早期检测意义较大。
Objective To investigate the clinic value of interleukin-6 (IL-6) in sepsis and septic shock. Method IL-6 concentrations in the peripheral blood of healthy or infected neonates were determined by ELISA. The expression of IL-6 mRNA were also detected by means or RT-PCR. Result IL-6 levels of infection group were significant higher than that of control group and IL-6 levels of septic shock group were the highest. All 5 neonates with sepsis had IL-6 mRNA expression and 3 of 6 control subjects had weak IL-6 mRNA expression. The optimal cut off point for diagnostic reference obtained with the percentile was 23 ng/L or more, allowing a sensitivity of 81%, and a specificity of 83%. Conclusion Increased IL-6 mRNA expression and serum IL-6 levels in the early stage of neonatal sepsis and septic shock are associated with the severity and outcome of illness. Determination of IL-6 levels seems to be an early indicator of neonatal bacterial infection.
出处
《临床军医杂志》
CAS
2001年第2期57-60,共4页
Clinical Journal of Medical Officers
