摘要
目的 :对中药锁阳中活性总多糖进行研究。方法 :SephadexG 10 0和G 15 0凝胶柱色谱纯化多糖 ;高效凝胶渗透色谱法测定多糖纯度和分子量 ;PC ,TLC及GC法测定中性糖组成 ;咔唑法测定糖醛酸含量。结果 :SYP A和SYP B的分子量分别为 3.1× 10 5和 2 .8× 10 5。中性单糖组成均为半乳糖、葡萄糖、阿拉伯糖、鼠李糖、甘露糖和核糖 ,摩尔比SYP A为 5 .1∶4.1∶1.6∶1.0∶0 .5∶0 .3,SYP B为 5 .2∶4.2∶1.5∶1.0∶0 .5∶0 .2。糖醛酸含量分别为 10 .7%和 10 .5 %。结论 :SYP A和SYP
Objective:To study the acidic heteropolysac charides of Cynomorium songaricum .Method:The polysaccharides were purified by Sephadex G 100 and G 150 gel column chromatography.Purity and molecular weight of the polysaccharides were determined by high performance gel permeation chromatography; neutral sugars composition were identified by PC, TLC and GC. Uronic acids were determined by carbazole method.Result:The molecular weight of SYP A and SYP B were estimated to be 3.1×10 5 and 2.8×10 5 respectively, and neutral sugars were composed of galactose, glucose, arabinose, rhamnose, mannose and ribose. The molar ratio for SYP A and SYP B were 5.1∶4.1∶1.6∶1.0∶0.5∶0.3 and 5.2∶4.2∶1.5∶1.0∶0.5∶0.2 respectively, The contents of uronic acids were 10.7% and 10.5% respectively.Conclusion:SYP A and SYP B are homogeneous acidic heteropolysaccharides.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2001年第6期409-411,共3页
China Journal of Chinese Materia Medica
