摘要
采用新型显微荧光分光光度系统 ,实现激光诱导人肺组织内源性显微荧光光谱与图像的测量 ,获得不同组织层 (即上皮层 ,粘膜层和软骨层 )的荧光分布和荧光特性 ,为探索癌与正常组织自体荧光光谱特征差异的根源 。
The microscopic fluorescence spectra and imaging of excised lung tissue sections were studied under a novel microspectrophotometer system using a He Cd laser light at 442 nm. Differences of the intrinsic autofluorescence distributions in different tissue layers, i.e., epithelium, mucosa and cartilage, were observed in normal and malignant lung tissues. The results show that the study of microscopic fluorescence on various tissue layers can be used to explore the origin of spectral differences between normal and abnormal lung tissues, which is very useful for the better understanding of the mechanism of the early cancer diagnosis using the laser induced autofluorescence technique.
出处
《中国激光》
EI
CAS
CSCD
北大核心
2001年第7期669-672,共4页
Chinese Journal of Lasers
基金
国家自然科学基金 (编号 :697780 2 9)
福建省科委国际科技合作计划资助项目
关键词
显微荧光分光光度系统
内源性显微荧光光谱
人肺组织
激光疗法
microspectrophotometer system, laser induced autofluorescence, microscopic fluorescence spectroscopy and imaging, human lung tissue
